USDA

Information on Classical Plant and Animal Breeding Activities Gao ID: GAO-07-1171R September 13, 2007

This report responds to a Congressional request for information on activities related to classical plant and animal breeding--creating an organism with desirable traits through controlled mating and selection without the insertion of genes from another species--that occurs at the U.S. Department of Agriculture (USDA). Within USDA, the Agricultural Research Service (ARS) and the Cooperative State Research, Education, and Extension Service (CSREES) are the primary scientific research agencies involved in classical plant and animal breeding activities. ARS has more than 100 research facilities in the United States and abroad and received about $1.3 billion in funding for fiscal year 2006. ARS conducts research to develop and transfer solutions to agricultural problems, and its research partners include universities; crop, horticultural, and livestock producer and industry organizations; state, federal, and other research agencies or institutions; private companies; and international agricultural research centers. CSREES, which received about $1.2 billion in funding for fiscal year 2006, has the primary responsibility for providing linkages between the federal and state components of a broad-based, national agricultural research, extension, and higher education system. As Congress has noted, classical breeding is important to agricultural producers as they seek to meet changing environmental conditions and shifting consumer demands. Congress raised concerns about the difficulty of quantifying public resources being dedicated to classical plant and animal breeding and asked us questions about these resources. Specifically, GAO was asked the following: (1) What USDA resources and personnel are devoted to classical plant and animal breeding activities, and what is USDA's budget for research and development of genetically engineered plant and animal varieties? (2) What is the total level of funding dedicated to USDA-funded extramural classical plant and animal breeding initiatives and research projects, and what are the specific initiatives and research projects? (3) What percentage of the overall USDA research budget goes to develop and release new, publicly held plant and animal varieties? What is the budget trend? (4) How many USDA-funded plant and animal breeders (scientist-years) using classical methods are there, and how many new varieties have they released in the last 2 years? (5) How many different varieties of nongenetically engineered or nonpatented corn, canola, soy, and cotton have been released and grown in the United States? (6) To what extent are breeding lines being imported from other countries? (7) How much public access is there to plant and animal germplasm? What barriers, if any, limit public access to germplasm?

In fiscal year 2005, the most recent year for which data are available, ARS and CSREES spent a total of about $145 million and 557 scientist-years on classical plant and animal breeding activities, according to USDA. ARS plant research funding for classical breeding, genomics, and genetic engineering generally trended upward from fiscal year 1997 to the mid-2000s, when the amount of funding leveled off for all three types of research. According to CSREES officials, in fiscal year 2005, the most recent year for which data are available, CSREES provided $31.7 million to fund classical plant breeding research projects and $26.2 million to fund classical animal breeding research projects. We were unable to determine the percentage of USDA's overall research budget devoted to these activities because CSREES does not track new, publicly held plant and animal varieties developed with its funding. CSREES explained that while it does track the percentage of its research budget devoted to projects that develop and release new plant varieties, it does not track whether these varieties become publicly or privately held. ARS officials said that in addition to its funding of classical plant breeding research, ARS contributes to the infrastructure for classical plant breeding in the United States by managing and making available to the public most of the seed stocks held by the U.S. government through the National Plant Germplasm System (NPGS)--primarily a federally and state-supported effort aimed at maintaining supplies of plant germplasm with diverse genetic traits for use in breeding and scientific research. ARS and CSREES expended a total of 557 scientist-years for classical plant and animal breeding research in fiscal year 2005, the last year for which data for both ARS and CSREES are available. CSREES officials told us that CSREES does not track the number of new varieties its grant recipients have released. ARS does, however, track the number of new varieties released. We were unable to determine the number of different varieties because USDA does not collect this information. While the amount of nongenetically engineered crops grown may not reflect on the number of different varieties of nongenetically engineered crops grown, USDA does maintain information on the percentage of acres of nongenetically engineered corn, cotton, and soybeans grown in the United States since 2000. USDA's National Agricultural Statistics Service conducts an annual national survey of 125,000 U.S. farmers on crops planted, including the number of acres planted with genetically engineered and nongenetically engineered corn, cotton, and soybeans. USDA does not collect information on breeding lines--genetic lines that provide the basis for modern varieties--imported from other countries. Although classical plant breeding researchers and USDA officials told us that the public generally has access to plant germplasm through ARS's NPGS, the researchers also said that most of NPGS's germplasm is not considered "elite" germplasm-- germplasm that is ready for a farmer to use. The available NPGS germplasm can require years of classical breeding research before it is ready for farmers to use. Regarding barriers to accessing plant germplasm, plant breeding researchers with whom we spoke said that intellectual property laws can limit access to elite germplasm. According to USDA officials, the public does not have access to animal germplasm because the purpose of the USDA's animal germplasm collection is conservation of the animal species for replacement of a breed, line, or strain if it is lost, or for research purposes of unique germplasm that would help characterize the breeds.



GAO-07-1171R, USDA: Information on Classical Plant and Animal Breeding Activities This is the accessible text file for GAO report number GAO-07-1171R entitled 'USDA: Information on Classical Plant and Animal Breeding Activities' which was released on September 15, 2007. This text file was formatted by the U.S. Government Accountability Office (GAO) to be accessible to users with visual impairments, as part of a longer term project to improve GAO products' accessibility. Every attempt has been made to maintain the structural and data integrity of the original printed product. Accessibility features, such as text descriptions of tables, consecutively numbered footnotes placed at the end of the file, and the text of agency comment letters, are provided but may not exactly duplicate the presentation or format of the printed version. The portable document format (PDF) file is an exact electronic replica of the printed version. We welcome your feedback. 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September 13, 2007: The Honorable Tom Harkin: Chairman: Committee on Agriculture, Nutrition, and Forestry: United States Senate: The Honorable Herb Kohl: Chairman: Subcommittee on Agriculture, Rural Development, Food and Drug Administration, and Related Agencies: Committee on Appropriations: United States Senate: Subject: USDA: Information on Classical Plant and Animal Breeding Activities: This report responds to your request for information on activities related to classical plant and animal breeding--creating an organism with desirable traits through controlled mating and selection without the insertion of genes from another species--that occurs at the U.S. Department of Agriculture (USDA). Within USDA, the Agricultural Research Service (ARS) and the Cooperative State Research, Education, and Extension Service (CSREES) are the primary scientific research agencies involved in classical plant and animal breeding activities. ARS has more than 100 research facilities in the United States and abroad and received about $1.3 billion in funding for fiscal year 2006. ARS conducts research to develop and transfer solutions to agricultural problems, and its research partners include universities; crop, horticultural, and livestock producer and industry organizations; state, federal, and other research agencies or institutions; private companies; and international agricultural research centers. CSREES, which received about $1.2 billion in funding for fiscal year 2006, has the primary responsibility for providing linkages between the federal and state components of a broad-based, national agricultural research, extension, and higher education system. CSREES provides funding for projects conducted in partnership with the state agricultural experiment stations, state cooperative extension system, land grant universities, colleges, and other research and education institutions. As you have noted, classical breeding is important to agricultural producers as they seek to meet changing environmental conditions and shifting consumer demands. You raised concerns about the difficulty of quantifying public resources being dedicated to classical plant and animal breeding and asked us questions about these resources. We found that generally, USDA data show a gradual increase in ARS classical plant breeding funding over the past 10 years, while funding for its classical animal breeding activities has remained level, with the exception of an upward trend from 2002 to 2004. USDA data also show that funding for CSREES classical plant breeding was higher in 1985 than in 1990, 1995, 2000, and 2005--the other 4 years for which USDA provided data. CSREES classical animal breeding funding data, however, show an upward trend since 1998. To answer your questions about classical breeding, we reviewed USDA data on funding and scientist-years[Footnote 1] devoted to classical breeding, genomics, and genetic engineering activities for plants and animals.[Footnote 2] We included genomics because some genomic techniques can be applied to both classical breeding and genetic engineering, according to USDA officials. In addition, we reviewed USDA extramural classical plant and animal breeding project information and statistics on the percentage of acres of U.S. farmland with genetically engineered corn, soybeans, and cotton.[Footnote 3] We also reviewed USDA data and spoke with USDA officials and university researchers, as well as with officials from agricultural nonprofit groups who also were farmers, on public access to germplasm and potential barriers to this access.[Footnote 4] We did not speak to a probability sample of these stakeholders; consequently, our results may not be representative of these groups. We conducted analyses to determine the reliability of USDA funding and scientist-years data and determined they were reliable for our purposes. For more information on our scope and methodology, see enclosure I. We conducted our work from April to September 2007 in accordance with generally accepted government auditing standards. Question 1: What USDA resources and personnel are devoted to classical plant and animal breeding activities, and what is USDA's budget for research and development of genetically engineered plant and animal varieties? In fiscal year 2005, the most recent year for which data are available, ARS and CSREES spent a total of about $145 million and 557 scientist- years on classical plant and animal breeding activities, according to USDA (see table 1). Table 1: USDA Funding and Scientist-Years Devoted to Classical Plant and Animal Breeding Activities, Fiscal Year 2005: Dollars in thousands. Plant Activities: Funding; ARS: $71,555[A]; CSREES: $31,688[B]; Total: $103,244. Plant Activities: Scientist-years; ARS: 155[A]; CSREES: 292[B]; Total: 447. Animal Activities: Funding; ARS: 15,111[C]; CSREES: 26,172[C]; Total: $41,284. Animal Activities: Funding; ARS: 33[C]; CSREES: 77[C]; Total: 110. Total: Funding; ARS: $86,667; CSREES: $57,861; Total: $144,527. Total: Scientist years; ARS: 188; CSREES: 369; Total: 557. Source: USDA. Note: Dollar figures have been adjusted for inflation to 2007 dollars and have been rounded to the nearest thousand dollars. [A] ARS classical plant breeding figures include funding used for germplasm evaluation--evaluation of plant materials for characteristics that can be utilized in plant genetic improvement and breeding heredity--and germplasm enhancement and plant breeding. [B] CSREES classical plant activities figures include funding for plant breeding activities and molecular-assisted plant breeding. While ARS separated molecular-assisted plant breeding into a genomics category, CSREES was unable to separate out this funding because it codes projects by strategic goal area, knowledge area, and field of science rather than by specific method used. Depending on the project, CSREES figures include either the total amount of the grant awarded or the amount that the recipient reported to CSREES as expended for the fiscal year. [C] ARS and CSREES classical animal breeding figures encompass all research at the whole-animal level, studying variation within and between lines and breeds of a species in poultry, beef and dairy cattle, sheep, swine, and aquaculture species. Depending on the project, CSREES figures include either the total amount of the grant awarded or the amount that the recipient reported to CSREES as expended for the fiscal year. [End of table] Shown below is the first of eight figures with information on USDA resources for classical breeding, genomics, and genetic engineering, broken out by agency (ARS or CSREES), type of resource (funding or scientist-years), and subject of research (plant or animal). ARS plant research funding for classical breeding, genomics, and genetic engineering generally trended upward from fiscal year 1997 to the mid- 2000s, when the amount of funding leveled off for all three types of research, as shown in figure 1. In particular, ARS funding devoted to classical plant breeding activities has consistently exceeded that devoted to genomics and genetic engineering over the past decade. Figure 1: ARS Plant Research Funding in 2007 Dollars, Fiscal Years 1997- 2007: [See PDF for image] Notes: Classical plant breeding figures include funding used for germplasm evaluation, germplasm enhancement, and plant breeding. Plant genomics figures include funding for research in molecular and cellular genetics, gene growth and development, genome mapping, and gene expression. Plant genetic engineering figures include funding for gene transformation and biotechnology risk assessment research. Source: USDA. [End of figure] Figure 2, in general, also shows a gradual upward trend in ARS scientist-years for classical breeding and genomics. The scientist- years for genetic engineering, however, have remained level since 2002. Figure 2: ARS Scientist-Years Dedicated to Plant Research, Fiscal Years 1997-2007: [See PDF for image] Notes: Classical plant breeding figures include germplasm evaluation, germplasm enhancement, and plant breeding. Plant genomics figures include funding for research in molecular and cellular genetics, gene growth and development, genome mapping, and gene expression. Plant genetic engineering figures include gene transformation and biotechnology risk assessment research. Source: USDA. [End of figure] Figure 3 shows CSREES plant research funding at five points in time from fiscal years 1985 to 2005. Because collecting the data was labor intensive and required manual review of thousands of project titles, we agreed with CSREES that data for 5 fiscal years would be sufficient for the purposes of this report. Specifically, the figure shows that funding for (1) classical breeding was higher in fiscal year 1985-- almost $35 million--than at the other four points; (2) genomic research was significantly higher in fiscal years 2000 and 2005 than in fiscal years 1985, 1990, and 1995; and (3) genetic engineering research was higher in fiscal year 2005 than in the previous selected fiscal years. CSREES officials commented that the increase for genetic engineering research was, in part, caused by the doubling of biotechnology risk assessment research funds between fiscal years 2000 and 2005. Figure 3: CSREES Plant Research Funding in 2007 Dollars, Fiscal Years 1985, 1990, 1995, 2000, and 2005: [See PDF for image] Notes: Data collected manually by CSREES. After sorting projects coded by knowledge area and field of science, CSREES staff manually reviewed titles of over 10,000 projects to provide 5 years of funding and scientist-year data. Classical plant breeding activities figures include funding for plant breeding activities and molecular-assisted plant breeding. Plant genomic figures include some of the research coded as plant genomic, genetics, and genetic mechanisms. Plant genetic engineering figures include funding for genetic transformation research. Depending on the project, CSREES's figures include either the total amount of the grant awarded or the amount that the recipient reported to CSREES as expended for the fiscal year. Source: USDA. [End of figure] Figure 4 shows CSREES plant research scientist-years at five points in time from fiscal years 1985 to 2005. The greatest number of scientist- years expended for classical plant breeding research was 411 in fiscal year 1985. However, the greatest number of scientist-years expended for both plant genomics and plant genetic engineering research was 167 and 41, respectively, in fiscal year 2005. Figure 4: CSREES Scientist-Years Dedicated to Plant Research, Fiscal Years 1985, 1990, 1995, 2000, and 2005: [See PDF for image] Source: USDA. Notes: Data collected manually by CSREES. After sorting projects coded by knowledge area and field of science, CSREES staff manually reviewed titles of thousands of projects to provide 5 years of funding and scientist-year data. Classical plant breeding activities figures include funding for plant breeding activities and molecular-assisted selection research. Plant genomic figures include some of the research coded as plant genomic, genetics, and genetic mechanisms. Plant genetic engineering figures include funding for genetic transformation research. [End of figure] Figure 5 shows similar funding levels for ARS classical animal breeding and animal genomics research from 1991 to 1998. This funding, however, increased more substantially for animal genomics than for classical animal breeding research since 1998. Figure 5 also shows that ARS animal genetic engineering research has remained relatively constant since 1998. Figure 5: ARS Animal Research Funding in 2007 Dollars, Fiscal Years 1991-2007: [See PDF for image] Source: USDA. Notes: Classical animal breeding figures encompass all research at the whole-animal level, studying variations within and between lines and breeds of a species in poultry, beef and dairy cattle, sheep, swine, and aquaculture species. Animal genomics figures include research at the molecular level of the genome. Animal genetic engineering figures include research that involves transferring genes between animal species. [End of figure] Figure 6 shows an upward trend in ARS scientist-years dedicated to genomic animal research since 1997 and an upward trend in ARS scientist- years dedicated to classical animal breeding research from 2002 to 2004, when it leveled off. Figure 6 also shows that ARS scientist-years dedicated to animal genetic engineering research have remained relatively constant since 1998. Figure 6: ARS Scientist-Years Dedicated to Animal Research, Fiscal Years 1997-2007: [See PDF for image] Source: USDA. Notes: Classical animal breeding figures encompass all research at the whole-animal level, studying variations within and between lines and breeds of a species in poultry, beef and dairy cattle, sheep, swine, and aquaculture species. Animal genomics figures include research at the molecular level of the genome. Animal genetic engineering figures include research that involves transferring genes between animal species. [End of figure] Figures 7 and 8 show an increase in funding and scientist-years in fiscal year 1998 for CSREES classical animal breeding. In addition, for animal genomics, funding and scientist-years also increased around the same time. However, in their explanation of this increase, agency officials commented that prior to 1998, under USDA's budget coding system, funding for classical breeding and genomics was not fully captured. They also stated that a change in the CSREES classification coding system in fiscal year 1998 resulted in more accurate figures. Funding and scientist-years for CSREES animal genetic engineering research have remained relatively flat since 1987, although CSREES did not have animal genetic engineering projects in fiscal years 1985, 1986, 1998, and 1999. Figure 7: CSREES Animal Research Funding in 2007 Dollars, Fiscal Years 1985-2005: [See PDF for image] Source: USDA. Notes: Classical animal breeding figures encompass all research at the whole-animal level, studying variation within and between lines and breeds of a species in poultry, beef and dairy cattle, sheep, swine, and aquaculture species. Animal genomics figures include research at the molecular level of the genome. Animal genetic engineering figures include research that involves transferring genes between animal species. Classical animal breeding and animal genomics data are from budget codes for fiscal years 1998-2005 and from a combination of USDA codes and manual reviews for fiscal years 1985-1997. All genetic engineering data are from a combination of USDA codes and manual reviews. Depending on the project, CSREES's figures include either the total amount of the grant awarded or the amount that the recipient reported to CSREES as expended for the fiscal year. [End of figure] Figure 8: CSREES Scientist-Years Dedicated to Animal Research, Fiscal Years 1985-2005: [See PDF for image] Source: USDA. Notes: Classical animal breeding figures encompass all research at the whole-animal level, studying variation within and between lines and breeds of a species in poultry, beef and dairy cattle, sheep, swine, and aquaculture species. Animal genomics figures include research at the molecular level of the genome. Animal genetic engineering figures include research that involves transferring genes between animal species. Classical breeding and genomics data are from budget codes for fiscal years 1998-2005 and from a combination of USDA codes and manual reviews for fiscal years 1985-1997. All genetic engineering data are from a combination of USDA codes and manual reviews. [End of figure] Question 2: What is the total level of funding dedicated to USDA-funded extramural classical plant and animal breeding initiatives and research projects, and what are the specific initiatives and research projects? According to CSREES officials, in fiscal year 2005, the most recent year for which data are available, CSREES provided $31.7 million to fund classical plant breeding research projects and $26.2 million to fund classical animal breeding research projects.[Footnote 5] (See encl. II for a list of USDA-funded extramural classical plant and animal breeding projects.) According to USDA officials, CSREES funds all USDA-funded extramural classical plant and animal breeding initiatives and research projects. Question 3: What percentage of the overall USDA research budget goes to develop and release new, publicly held plant and animal varieties? What is the budget trend? We were unable to determine the percentage of USDA's overall research budget devoted to these activities because CSREES does not track new, publicly held plant and animal varieties developed with its funding. CSREES explained that while it does track the percentage of its research budget devoted to projects that develop and release new plant varieties, it does not track whether these varieties become publicly or privately held. According to CSREES, one reason for this is that the variety development process is longer than the federal grant authorization cycle. Although we could not determine the percentage of the overall USDA research budget that goes to develop and release new, publicly held plant and animal varieties, according to ARS data, 10.7 percent of ARS's fiscal year 2007 $1.2 billion budget is to be directed toward these activities. ARS officials said that in addition to its funding of classical plant breeding research, ARS contributes to the infrastructure for classical plant breeding in the United States by managing and making available to the public most of the seed stocks held by the U.S. government through the National Plant Germplasm System (NPGS)--primarily a federally and state-supported effort aimed at maintaining supplies of plant germplasm with diverse genetic traits for use in breeding and scientific research. Funding for NPGS, shown in figure 9, increased from $24.5 million in fiscal year 1996 to $46.1 million in fiscal year 2003.[Footnote 6] ARS also supports crop genome databases that enable researchers to access and leverage emerging information for breeding and development of gene markers. ARS officials call these databases plant bioinformatics. Funding information for plant bioinformatics goes back only to fiscal year 2002. Figure 9: ARS Funding for Classical Plant Breeding, National Plant Germplasm System, and Plant Bioinformatics in 2007 Dollars, Fiscal Years 1992-2007: [See PDF for image] Source: USDA. [End of figure] ARS generally is not involved with the development of publicly held animal varieties. However, in recent years, it has conducted some classical animal breeding research to create genetically improved lines of fish germplasm in aquaculture, specifically for catfish. The funding trend for this research, which has been mostly flat since 2003, is shown in figure 10. Figure 10: ARS Funding for Fish Germplasm Research in the Aquaculture Area in 2007 Dollars, Fiscal Years 1999-2007: [See PDF for image] Source: USDA. [End of figure] Question 4: How many USDA-funded plant and animal breeders (scientist- years) using classical methods are there, and how many new varieties have they released in the last 2 years? ARS and CSREES expended a total of 557 scientist-years for classical plant and animal breeding research in fiscal year 2005, the last year for which data for both ARS and CSREES are available, as shown in table 1. CSREES officials told us that CSREES does not track the number of new varieties its grant recipients have released. ARS does, however, track the number of new varieties released. As figure 11 shows, ARS released 12 to 52 new plant varieties a year between fiscal years 1988 and 2006. In the last 2 years (fiscal years 2005 and 2006), ARS released a total of 54 new plant varieties. In addition, ARS commented that since fiscal year 1998, it has released 37 to 311 plant germplasm lines per year, which allowed other U.S. classical plant breeders to incorporate improved traits into their locally adapted varieties. ARS also released two new animal varieties, both catfish, through its aquaculture research in the past 2 years. Eight scientist-years were involved in this aquaculture research. Figure 11: Number of Plant Varieties ARS Has Released, Fiscal Years 1988-2006: [See PDF for image] Source: USDA. [End of figure] Question 5: How many different varieties of nongenetically engineered or nonpatented corn, canola, soy, and cotton have been released and grown in the United States? We were unable to determine the number of different varieties because USDA does not collect this information. While the amount of nongenetically engineered crops grown may not reflect on the number of different varieties of nongenetically engineered crops grown, USDA does maintain information on the percentage of acres of nongenetically engineered corn, cotton, and soybeans grown in the United States since 2000. USDA's National Agricultural Statistics Service conducts an annual national survey of 125,000 U.S. farmers on crops planted, including the number of acres planted with genetically engineered and nongenetically engineered corn, cotton, and soybeans. Figure 12 shows the downward trend in nongenetically engineered crops since 2000. Figure 12: Percentage of Acres on Which Nongenetically Engineered Corn, Cotton, and Soybeans Were Grown in the United States, Years 2000-2007: [See PDF for image] Source: USDA. Note: According to the National Agricultural Statistics Service, data from farmers surveyed in 48 states represent 81 percent to 86 percent of all corn-planted acres, 89 percent to 90 percent of all soybean- planted acres, and 81 percent to 92 percent of all upland cotton- planted acres. [End of figure] Question 6: To what extent are breeding lines being imported from other countries? USDA does not collect information on breeding lines--genetic lines that provide the basis for modern varieties--imported from other countries. Question 7: How much public access is there to plant and animal germplasm? What barriers, if any, limit public access to germplasm? Although classical plant breeding researchers and USDA officials told us that the public generally has access to plant germplasm through ARS's NPGS, the researchers also said that most of NPGS's germplasm is not considered "elite" germplasm--germplasm that is ready for a farmer to use. The available NPGS germplasm can require years of classical breeding research before it is ready for farmers to use, according to the researchers with whom we spoke. One USDA official told us that ARS plant varieties and improved germplasm lines are publicly released and can be obtained from ARS researchers. According to ARS, in 2006, NPGS received 6,662 requests for one or more genetic material samples (accessions) and responded by distributing 159,266 of these samples. When the supply of a requested seed becomes limited, according to a USDA official, NPGS either provides a small amount or delays filling the request until sufficient seeds are regenerated. Regarding barriers to accessing plant germplasm, plant breeding researchers with whom we spoke said that intellectual property laws can limit access to elite germplasm. For example, the University and Small Business Patent Procedures Act, also known as the Bayh-Dole Act, allowed nonprofit organizations, such as universities, to retain title to and market the inventions they created using federal research funds.[Footnote 7] As a result, universities now restrict their sharing of germplasm, according to these plant breeding researchers. According to USDA officials, the public does not have access to animal germplasm because the purpose of the USDA's animal germplasm collection is conservation of the animal species for replacement of a breed, line, or strain if it is lost, or for research purposes of unique germplasm that would help characterize the breeds. Access to germplasm is determined on a case-by-case basis by species committees that consist of university and federal scientists and industry representatives. Some of the animal germplasm samples were donated by private industry under terms that prevent access to the samples for a limited amount of time. Agency Comments: We provided a draft of this report to USDA for review and comment, and USDA provided us with oral comments. USDA generally agreed with the contents of the draft report. The department provided us with technical comments, which we incorporated into the report as appropriate. As agreed with your offices, unless you publicly announce the contents of this report earlier, we plan no further distribution until 30 days from the date of this report. At that time, we will send copies to the Secretary of Agriculture, interested congressional committees, and other interested parties. We will also make copies available to others upon request. In addition, the report will be available at no charge on the GAO Web site at [hyperlink, http://www.gao.gov]. If you or your staffs have any questions about this report, please contact me at (202) 512-3841 or shamesl@gao.gov. Contact points for our Offices of Congressional Relations and Public Affairs may be found on the last page of this report. Key contributors to this report were José Alfredo Gómez, Assistant Director; Allen Chan; Nancy Crothers; Kevin Bray; and Greg Wilmoth. Signed by: Lisa Shames: Director, Natural Resources: and Environment: Enclosures: Enclosure I: Scope and Methodology: To determine what U.S. Department of Agriculture (USDA) resources and scientist-years are devoted to classical breeding, genomics, and genetic engineering activities for plants and animals, we asked USDA to provide us with funding and scientist-years data since 1985. The Agricultural Research Service (ARS) gave us these data from its budget codes. We reported ARS animal research funding data from fiscal years 1991 to 2007 because ARS did not have animal research funding data for the years before fiscal year 1991 that it felt were sufficiently accurate to report. We reported ARS plant research funding data from fiscal years 1997 to 2007 because ARS did not have a separate budget code for genomics before fiscal year 1997. Data for classical breeding and genomic research before fiscal year 1997 were combined into one budget code. The Cooperative State Research, Education, and Extension Service (CSREES) provided plant research data from its current research information system. Using this database, CSREES sorted projects coded by knowledge area and field of science. CSREES staff then manually reviewed titles of over 10,000 projects to provide 5 years (fiscal years 1985, 1990, 1995, 2000, and 2005) of funding and scientist-year data. CSREES animal research data for classical breeding and genomics for fiscal years 1985 to 1997 are from a combination of USDA codes and manual CSREES reviews. Similar data for fiscal years 1998 to 2005 are from budget codes. CSREES animal research data for genetic engineering are from a combination of USDA codes and manual CSREES reviews. To determine the total overall level of funding dedicated to USDA- funded extramural classical plant and animal breeding initiatives and research projects and the specific names of the initiatives and research projects, we obtained project funding information and a list of project names from CSREES. (See encl. II.) We did not review individual USDA projects to ensure the accuracy of the list. To determine the amount of USDA funding that goes to develop and release new, publicly held plant and animal varieties, we obtained funding figures for ARS's National Plant Germplasm System, plant bioinformatics program, and fish germplasm research in the aquaculture area. We obtained the number of scientist-years involved in developing and releasing new plant and animal varieties and the number of new varieties released from ARS. CSREES does not track this information. To determine the reliability of ARS and CSREES funding and scientist- years data and of ARS data on the number of new varieties it has released, we examined existing information about the data and systems that produced them, questioned knowledgeable agency officials about the data, and discussed the manual coding process with CSREES. While we determined that these data were reliable for our purposes, we did not review whether USDA had accurately categorized the funding and scientist-years data. We were unable to determine how many different varieties of nongenetically engineered or nonpatented corn, canola, soy, and cotton have been released and grown in the United States because USDA did not have this information. However, USDA provided us with survey statistics on the percentage of acres of U.S. farmland with genetically engineered corn, soybeans, and cotton. USDA did not have this information for canola or information on the percentage of nonpatented crops grown in the United States. The survey statistics were taken from USDA's annual June Agricultural Survey, which surveyed more than 125,000 farmers about their crops. We were unable to answer the extent to which breeding lines were imported into the United States because USDA did not have this information. To determine how much public access there is to germplasm and what barriers, if any, limit public access, we spoke with USDA officials, university breeding researchers, and officials from agricultural nonprofit groups who were also farmers. We did not speak to a probability sample of these stakeholders; consequently, our results may not be representative of these groups. We conducted our work from April to September 2007 in accordance with generally accepted government auditing standards. [End of section] Enclosure II: CSREES Classical Plant and Animal Breeding Projects: CSREES provided the following list of extramural classical plant breeding projects for fiscal year 2005. Projects with funding to multiple states may be repeated on this list. 1. Development and Management of Canola in the Great Plains Region. 2. Evaluation of Native and Exotic Herbs and Vegetables for Their Production Potentials, Stress Physiology, and Nutritional Qualities. 3. Development of Winter Type Canola Lines for the Mid-South United States. 4. Development of New Commercial Fruit Crops for Kentucky and the Southeastern United States. 5. Improving Sweet Potato Production in Alabama through Breeding, Selection, and Biotechnology Techniques. 6. Characterizing Soybean and Corn Genotypes for Phosphorus Hyperaccumulation. 7. Development of White Lupin as an Alternative Crop in Virginia. 8. Improving Sicklepod for Industrial and Medicinal Uses by Means of Conventional and Molecular Breeding Approaches. 9. Improving Sweet Potato Production in Limited Resource Farming Systems through Cultivar Development and Integrated Pest Management. 10. Breeding Aroids for Quality, Productivity, and Disease/Pest Resistance with Emphasis on Anthurium. 11. Conservation, Management, Enhancement, and Utilization of Plant Genetic Resources. 12. Breeding, Evaluation, and Selection of Hardy Landscape Plants. 13. Wheat and Oat Genetics and Breeding. 14. Oat Breeding and Genetics. 15. Barley Breeding and Genetics. 16. Plant Genetic Resource Conservation and Utilization. 17. Conservation and Utilization of Plant Genetic Resources. 18. Improvement of Winter Wheat through Breeding. 19. Genetics, Breeding, and Physiology of Yield in Cucurbits. 20. Breeding and Testing Improved Varieties of Spring Barley, Wheat, and Oats. 21. Plant Genetic Resource Conservation and Utilization--Colorado State University. 22. Genetics, Breeding, and Evaluation of Winter Small Grains Crops for Nebraska. 23. Breeding and Testing Oats, Barley, and Canola for Michigan. 24. Plant Genetic Resource Conservation and Utilization--Texas A&M University. 25. Plant Genetic Resource Conservation and Utilization--Oregon State University. 26. Genetics and Breeding of Alfalfa for New Uses and Forage Quality. 27. Genetic Manipulation of Sweet Corn Quality and Stress Resistance. 28. Tree Fruit and Grape Investigations. 29. Breeding and Genetics of Hazelnut. 30. Development of Germplasm and Breeding Methods for the Improvement of Tomato. 31. Breeding and Genetics of Floricultural Crops: Germplasm Enhancement, Risk Assessment of Invasiveness Potential. 32. Conservation, Management, Enhancement, and Utilization of Plant Genetic Resources. 33. Development of New Potato Cultivars for Colorado via Germplasm Enhancement and Evaluation. 34. Wild Perennial Glycine Information Management and Untilization. 35. Conservation and Utilization of Plant Genetic Resources. 36. Development of Cotton Germplasm/Cultivars with Resistance to Biotic and Abiotic Stresses. 37. Evaluation and Genetic Improvement of Forage Legumes. 38. Genetic Improvement of Beans (Phaseolus Vulgaris L.) for Yield, Pest Resistance, and Food Value. 39. Genetic Manipulation of Sweet Corn Quality and Stress Resistance. 40. Rootstock and Interstem Effects of Pome and Stone Fruit Trees. 41. Genetic Improvement of Beans (Phaseolus Vulgaris L.) for Yield, Pest Resistance, and Food Value--Oregon State University. 42. Genetic Improvement of Beans (Phaseolus Vulgaris L.) for Yield, Pest Resistance, and Food Value--Cornell University. 43. Genetic Improvement of Beans (Phaseolus Vulgaris L.) for Yield, Pest Resistance, and Food Value--Colorado State University. 44. Genetic Improvement of Beans (Phaseolus Vulgaris L.) for Yield, Pest Resistance, and Food Value--University of California. 45. Improvement of Forage Quality in Alfalfa by Breeding. 46. Genetic and Breeding of Cool Season Vegetable Crops. 47. Improvement of Cotton Cultivars Adapted to Stripper Harvesting and Short Growing Season. 48. Vaccinium Breeding and Genetics. 49. Development of Improved Vegetable Legume Varieties for the Southwest. 50. Quantitative Genetics and Cultivar Development. 51. The Impact of Hybridization on Plant Population Genetics and Ecology. 52. Alfalfa Breeding. 53. Genetic Conversion of Exotic Sorghums for Temperate Zone Use. 54. Development of Improved Potato Varieties for Texas and the Southwest. 55. Development of Disease-Resistant Wheat Germplasm and Studies of Selected Wheat Diseases. 56. Breeding Superior Raspberry Cultivars for the Pacific Northwest. 57. Prunus Genetics, Germplasm, and Cultivar Development for Mild Winter Zones. 58. Fresh-Market Tomato Breeding and Genetics. 59. Use of Genetic Resistance to Control Leaf Blight and Ear Rot Diseases of Corn. 60. Barley Breeding and Genetics. 61. Utilization of Species of Arachis to Improve Cultivated Peanuts. 62. Breeding and Genetics of Barley. 63. Breeding Superior Strawberry Cultivars for the Pacific Northwest. 64. Genetic Improvement of Beans (Phaseolus Vulgaris L.) for Yield, Disease Resistance, and Food Value. 65. Sorghum Breeding and Genetics. 66. Insect Resistance and Stress Adaptation through Genetic Manipulation of Sorghum. 67. Cucumber, Luffa, and Watermelon Breeding and Genetics. 68. Enhancement of Arachis Germplasm to Improve Peanut (A. Hypogaea L.) Cultivars. 69. Stone Fruit Breeding for New York Tree Fruit Industry Diversification. 70. Genetics and Varietal Improvement of Strawberries. 71. Genetics, Breeding, and Evaluation of Citrus Fruits. 72. Disease Resistance in Peanut to Sclerotinia Blight. 73. Rootstock and Interstem Effects on Pome and Stone Fruit Trees. 74. Spring Wheat Breeding and Genetics. 75. Improvement and Testing of Winter Small Grains. 76. Breeding and Genetics of Peach. 77. Abiotic Stress Tolerance in Plants. 78. Small Grains Breeding Investigations. 79. Rootstock and Interstem Effects on Pome and Stone Fruit Trees. 80. Develop Management Practices for Recently Introduced Rice Diseases in California. 81. Improvement of Quality and Performance of Colorado Wheat. 82. Novel Methods for Soybean Genetic Improvement and Genomic Analysis. 83. Vegetable Breeding Material Evaluation and Alternative Crop Development. 84. Molecular Population Genetics of Natural Populations. 85. Genetic Improvement of Bean (Phaseolus Vulgaris L.) for Yield, Pest Resistance, and Food Value. 86. Peanut Breeding and Genetics. 87. Development of Multiple-Use Barley Varieties. 88. Winter Wheat Breeding Program. 89. Breeding Annual Ryegrass for Forage and for Turf. 90. Identification, Propagation, and Development of Ornamentals and Floriculture Plants for Texas. 91. Utilization of Forest Genetic Resources to Enhance Productivity of Forested Lands. 92. Forage and Turf Grass Breeding and Genetics. 93. How Populations Cope with Heterogeneous Environments: Plasticity, Adaptation, and Population Coexistence. 94. Winter Wheat Breeding and Genetics. 95. Genetic Improvement of Apple. 96. Breeding Cotton Varieties for North Carolina. 97. Vegetable Improvement for Appearance, Flavor, Texture, Nutrition, and Health Benefits. 98. Improvement of Plant Defenses Against Botrytis Cinerea. 99. Vegetable Breeding and Genetics. 100. Genetic Improvement of Peach and Almond. 101. Genetic Manipulation of Sweet Corn and Quality and Stress Resistance. 102. Genetic Diversity and the Propagation of Native Hawaiian Plants for the Ornamentals Industry. 103. Testing and Evaluation of Berry Crops for Commercial Production in the Pacific Northwest. 104. Genetic Improvement of Walnut. 105. Multidisciplinary Evaluation of New Apple Cultivars. 106. Evaluation of Soybean Cultivars and Advanced New Strains and Corn and Grain Sorghum Hybrids in Arkansas. 107. Nursery and Greenhouse Production of Ornamentals with Emphasis on Roses and Bedding Plants. 108. Ecology And Management of European Corn Borer and Other Stalk- Boring Lepidoptera. 109. Use of Wild Lycopersicon Species in Breeding for Improvement of Cultivated Tomato. 110. Soybean Breeding and Genetic Studies. 111. Breeding and Genetics of the Small Grains Cereals. 112. Breeding, Genetic, and Agronomic Studies of Barley in California. 113. Breeding and Genetics for the Improvement of Potato (Solanum Tuberosum L.) for Yield, Quality, and Pest Resistance. 114. Genetic Improvement of Strawberries and Blueberries. 115. Genetic Improvement of Beans (Phaseolus Vulgaris L.) for Yield, Disease Resistance, and Food Value. 116. Genetic Manipulation of Sweet Corn Quality and Stress Resistance. 117. Breeding and Genetics of Winter Wheat. 118. Wheat Breeding and Genetics. 119. Genetic Improvement of Sorghum Bicolor (L) Moench for Improved Productivity, Adaptability, and Quality. 120. Development of New Potato Clones for Environmental and Economical Sustainability. 121. Introduction and Evaluation of Ornamental Plants. 122. Wheat Breeding and Molecular Genetics. 123. Conservation and Utilization of Germplasm at the C.M. Rick Tomato Genetics Resource Center. 124. Mitigation of Diseases of Dry Edible Bean and Stem Rot of Soybean by Managed Plant Resistance. 125. Developing New Apple Cultivars for Washington State. 126. Turfgrass Breeding, Genetics, and Cultivar Development. 127. Breeding and Development of Buffalograss for the Central Great Plains. 128. Breeding and Genetics of Corn. 129. Breeding Tree Fruits Adapted to the Soils and Climate of Arkansas. 130. Breeding and Genetics of Small Fruits and Grapes in Arkansas. 131. Evaluation, Development, and Management of Native and Adapted Grass Species for Turfgrass Applications in the Intermountain West. 132. Breeding Multiple Stress Tolerant Corn for Texas Conditions. 133. Potato Breeding and Genetics. 134. Evaluation and Identification of Potential Turfgrass Species for Lower Latitude Turf. 135. Feed Barley for Rangeland Cattle. 136. Hawaii Floriculture Research Grant--2005. 137. Grass Seed Cropping Systems for a Sustainable Agriculture: ID, OR, and WA. 138. Development of Phytophthora Root Rot-Resistant Avocado Rootstocks for the Caribbean. 139. Russian Wheat Aphid Resistance, Stress Tolerance, and Quality Enhancement of Wheat. 140. Life and Death in Plants: Studies on Perennial Wheat as a Sustainable Alternative Cropping System. 141. Selecting and Breeding Sweet Potato Genotypes Under Minimum Cultural Conditions. 142. Developing Alternative Vegetable Soybean Crops to Farmers. 143. Breeding Vegetables for Pest and Stress Tolerance. 144. Breeding and Genetics of Herbaceous and Woody Landscape Plants. 145. Enhancing Impatiens Resistance to Feeding by Western Flower Thrips. 146. Intermediate Stage Evaluation of Apple Rootstocks for the Eastern U.S. 147. Forage Germplasm Evaluation in Louisiana. 148. Selection, Development, and Propagation of Native Herbaceous Landscape Plants. 149. Breeding, Genetics, and Productivity of Small Grains. 150. Small Grain Genetic Improvement, Variety Testing, and Cultural Practices. 151. Turfgrass Breeding and Evaluation. 152. Strawberry Cultivar Development. 153. Genetic Improvement of Wheat. 154. Improvement of Hard Winter Wheats and Other Small Cereal Grains for Kansas. 155. Increased Genetic Diversity for the Winter Wheat Breeding Program in Oklahoma. 156. Selection and Evaluation of Superior Woody Ornamental Plants Suitable for Arkansas Landscapes. 157. Soybean Breeding and Genetics. 158. Breeding and Evaluation of Improved Soybean Cultivars and Germplasm. 159. Breeding and Genetics of Temperate Forage Grasses and Legumes. 160. Genetic Diversity in Cotton through Germplasm Enhancement and Molecular Genetics. 161. Breeding Tropical Vegetable Crops. 162. Analysis of Disease Resistance in Rice. 163. Improving Spring Wheat Varieties for the Pacific Northwest. 164. Breeding and Genetics of Cabbage, Broccoli, Cauliflowers, and Common Beans. 165. Development of Adapted Potato Varieties for the Mid-Atlantic and Southeastern United States. 166. Sweet Potato Breeding and Genetic Enhancement. 167. Screening Genotypes for Traits Associated with Tolerance to Abiotic Stress for Crops Grown in the Southern Plains. 168. Genetic Variability, Selection, and Inbreeding in Flower Crops. 169. Improvement of Fruit Size and Yield of Mandarins in California and Genetic Analyses of Date Palms and Ornamental Foliage Plants. 170. Variety Evaluation of Corn, Corn Silage, Cotton, Small Grain, and Soybean. 171. Multidisciplinary Evaluation of New Apple Cultivars. 172. Soybean Genetic Improvement and Cultivar Evaluation in Louisiana. 173. Vegetable Crop Studies. 174. Multidisciplinary Evaluation of New Apple Cultivars. 175. Improving Landscape and Horticultural Production Systems. 176. Quantitative Genetics with Focus on Corn Breeding and Corn Germplasm Improvement. 177. Improvement of Strawberry and Raspberry Cultivars. 178. Development of Disease Management Strategies for Soybean Pathogens in Ohio. 179. Genetic Improvement of Beans (Phaseolus Vulgaris L.) for Yield, Disease Resistance, and Food Value. 180. Development of Snap Bean Varieties and Genetic Investigations in Common Bean. 181. Breeding Sorghum for Improved Yield Potential and Stress Tolerance. 182. Genetic Manipulation of Sweet Corn Quality and Stress Resistance. 183. Sugercane Improvement for Arid, Alkaline Environments. 184. Molecular Mapping and Marker-Assisted Selection and Breeding for Disease Resistance and Improved Fruit Quality in Tomato. 185. Development of High-yielding, Multiple Pest-Resistant Soybean Breeding Cultivars with Improved Nutritional Value for Missouri. 186. Breeding and Evaluation for Improved Rice Varieties. 187. Breeding and Testing of Winter Grain Crops. 188. Genetic Improvement of Floricultural Crops. 189. Enhancing Soybean Production-Efficiency and Stability through Breeding and Genetics. 190. Genetic Improvement of Melons, Peppers, and Tomatoes to Enhance Production and Quality in Texas. 191. Production Strategies for Improved Vegetable Production and Alternative Crops for Diversification. 192. Evaluation of Hard Red Spring and Hard White Spring Wheat Quality in Relation to End-Use Functionality. 193. Genetic Improvement of Beans (Phaseolus Vulgaris L.) for Yield, Pest Resistance, and Food Value. 194. Genetic Improvement of Beans (Phaseolus Vulgaris L.) for Yield, Pest Resistance, and Food Value. 195. Genetic and Turfgrass Breeding. 196. Wheat Breeding and Small Grain Management for the High Rainfall Area of East Texas. 197. Evaluation of Selected Fruit Species for Adaptation to Southern Louisiana. 198. Breeding for Disease Resistance and Processing Qualities of Potato: Determination of Genetic Variability of Pathogens and Disease Management. 199. Genetic Improvement of Underutilized Perennial Crop Plants. 200. Cereal Breeding. 201. Developing Land Races Adapted to Ohio Landscapes. 202. Regional Moderate-Chilling Peach and Nectarine Breeding and Evaluation Project. 203. Winter Wheat Breeding and Genetics. 204. Selection and Adaptation of Grass and Legume Species for Forage Production in the Southern Coastal Plain and Penisular Florida. 205. Selection and Adaptation of Grass and Legume Species for Forage Production in the Southern Coastal Plain and Penisular Florida. 206. Development of Genetic Resources for Cotton. 207. Genetic Manipulation of Sweet Corn Quality and Stress Resistance. 208. Improving Wheat Quality in the State of Washington. 209. Development of Genetic Resources for Cotton. 210. Strawberry Breeding and Genetics. 211. Development of Genetic Resources for Cotton. 212. Development of Blueberry Cultivars Adapted to the Deep South. 213. Development of Genetic Resources for Cotton. 214. Potato Variety Improvement, Evaluation, Management, and Seed Increases in Idaho. 215. Genetic Studies and Germplasm Enhancement in Cool-Season Legumes. 216. Maize Breeding and Germplasm Base-Broadening. 217. Development of Improved Wheat Cultivars for Idaho. 218. Molecular Genetic Accelerated Development of Red-Skinned, Golden Nematode-Resistant Potato Varieties. 219. Studies of Host-Parasite Interactions between Small Grains and Their Fungal Pathogens. 220. Development of Cornus Florida Cultivars Resistant to Dogwood Anthracnose and Powdery Mildew. 221. Integrated Tree Fruit Physiology, Genetics, and Management. 222. Breeding Cotton Germplasm with Higher Lint Yield, Improved Fiber Quality, and Resistance to Biotic and Abiotic Stresses. 223. Breeding Southern Peas. 224. Breeding, Disease Epidemiology, Pathogen Characterization, and Genetic and Molecular Determination of Disease; Resistance in Spinach. 225. Breeding Improved Wheat Cultivars and Germplasm for Ohio. 226. Exotic Germplasm Conversion and Breeding Common Bean (Paswoulus Vulgaris L.) for Resistance to Abiotic and; Biotic Stresses. 227. Rice Breeding and Cultivar Development in Mississippi. 228. Development of Genetic Resources for Cotton. 229. Cultivar Testing, Breeding, and Culture of Vegetables. 230. Breeding and Genetics of Barley and Wheat for Increased Productivity, Value, and Durability. 231. Variety and Quality Evaluation of Virginia-Type Peanuts. 232. Preservation, Characterization, and Genetic Improvement of Hawaiian Taro. 233. Variety and Quality Evaluation of Virginia-Type Peanuts. 234. Developing Biotic and Abiotic Stress Tolerance in Corn. 235. Maize Genetics and Improvement. 236. Breeding High-yielding, High-value Soybean for South Carolina. 237. Genetic Improvement of Soybean for Food Value, Yield, and Pest Resistance. 238. Integrated Disease Management of Dry Edible Beans in North Dakota. 239. Disease Resistance in Small Grain Cereal Crops and Their Wild Relatives. 240. Salinity and Environmental Stress Resistance in Turfgrass and Landscape Plants for Recycled Water Irrigation; and Phytoremediation. 241. Peanut Breeding and Genetics. 242. Development of Superior All-Male Asparagus Hybrids for All Major Cultivation Regions. 243. Development of Cotton Cultivars and Breeding Lines Adapted in Mississippi. 244. Utilization of Monoploid Derivatives of Potato in Genetic Studies. 245. Specialty Crop Evaluation and Product Development. 246. Biology and Control of Leaf Rust and Spring Dead Spot in Wheat and Bermudagrass Respectively Intergrated Activity. 247. Cotton Germplasm Breeding for Improved Lint Yield, Fiber Quality, and Resistance to Biotic and Abiotic Stresses. 248. Peanut Breeding and Management. 249. Breeding and Germplasm Enhancement for New Jersey Cranberry and Blueberry Industries. 250. Evaluating Salt Tolerance and Seed Germination in New Guayule Breeding Lines. 251. Development of Multiple Disease-Resistant Commercial Tomatoes. 252. Development of Wheat Varieties Adapted to Oregon with Improved Disease Resistance, Stress Tolerance, and; Superior End-Use Properties. 253. Improved Sugarcane Cultivars for Louisiana through the Identification of Superior Parents, Crosses, and Seedlings. 254. New Crop Development for Oregon Agriculture, with Current Emphasis on Meadowfoam (Limnanthes). 255. Breeding and Genetics of Forage Crops to Improve Productivity, Quality, and Industrial Uses. 256. Development and Utilization of DNA Markers for Soybean Breeding and Cultivar Development for North Carolina Environments. 257. Market-Targeted Breeding with Molecular Wheat Protein Quality Assessment. 258. Developing and Managing New Potato Varieties. 259. Onion Breeding: Research and Development for Onion Improvement. 260. Characterizing Genetic and Biochemical Differences among American Ginseng Populations. 261. Woody Landscape Plant Breeding, Evaluation, and Introduction Program. 262. Oat (Avena Sativa) Cultivar Improvement. 263. Development of New Gene Combinations for Cotton Improvement. 264. Development of New Potato Clones for Improved Pest Resistance, Marketability, and Sustainability in the East. 265. Developing a New Hybrid Breeding System for Alfalfa. 266. Development of New Potato Clones for Improved Pest Resistance, Marketability, and Sustainability in the East. 267. Breeding and Genetics of Forage Crops to Improve Productivity, Quality, and Industrial Uses. 268. Potato Breeding and Cultivar Development. 269. Development of New Potato Clones for Improved Pest Resistance, Marketability, and Sustainability in the East. 270. Breeding Pierce's Disease-Resistant Table and Raisin Grapes. 271. Development of New Potato Clones for Improved Pest Resistance, Marketability, and Sustainability in the East. 272. Breeding and Genetics of Forage Crops to Improve Productivity, Quality, and Industrial Uses. 273. Cotton Germplasm Improvement and Genetics Research. 274. Development of New Potato Clones for Improved Pest Resistance, Marketability, and Sustainability in the East. 275. Corn (Zea Mays L.) Breeding in the Northern Corn Belt. 276. Applied Sweet Potato Genomics. 277. Genetic Improvement of Alfalfa (Medicago Sativa L.) Germplasm for New Mexico. 278. Genetic Improvement of Forage Grass and Legume Species. 279. Cultivar Development and Genetic Improvement of Oilseed Rape. 280. Improvement of Proso Millet and Other Crops for Adaptation to Western Nebraska. 281. Breeding and Genetics of Forage Crops to Improve Productivity, Quality, and Industrial Uses. 282. Genomic Tools for Peach and the Rosaceae. 283. Breeding and Genetics of Forage Crops to Improve Productivity, Quality, and Industrial Uses. 284. Breeding and Testing Vegetable Crops for Processing, Fresh Market, and Home Garden. 285. Development of Rice Germplasm Using Molecular and Conventional Genetic Approaches. 286. Developing Hard White Spring Wheat, Specialty Wheat, and Sawfly Resistant Wheat. 287. Biological Improvement, Habitat Restoration, and Horticultural Development of Chestnut by Management of Populations,; Pathogens, and Pests. 288. Breeding and Genetics of Flax. 289. Breeding and Genetics of Forage Crops to Improve Productivity, Quality, and Industrial Uses. 290. Genetic Manipulation of Sweet Corn Quality and Stress Resistance. 291. Improving Corn Silage Wheat and Barley Production in Pennsylvania. 292. Use of Genetic Resistance to Control Aflatoxin and Fumonisin in Corn Grain. 293. Soybean Breeding and Production. 294. Domestication of Western Vaccinium Species (Bilberries, Blueberries, Cranberries, Huckleberries, and Whortleberries). 295. Development of Stress-Resistant/High-yield Sorghum Germplasm for Release and Use in Investigation of Contributing; Physiological Mechanisms. 296. Breeding Cotton for Adaptation to Arkansas Conditions. 297. Quantitative Genetics and Crop Breeding Investigations. 298. Onion Genetic Improvement. 299. Breeding and Genetics of Legumes for Use as Forage and in Sustainable Agriculture. 300. Genetic Improvement of Forage and Cover Crop Species. 301. Genetic Improvement of Chile (Capsicum) Germplasm for New Mexico. 302. Spring Wheat Breeding and Genetics. 303. Evaluation of Native and Naturalized Germplasm for Reduced-Input Turfgrass in the Northern Plains. 304. Breeding Perennial Grasses and Legumes for Forage, Biomass, Wildlife Habitat, Conservation, and Tolerance to Stresses. 305. Development of Oat Varieties for South Dakota. 306. Wheat Germplasm Enhancement. 307. Golden Nematode Resistant Chipping and Tablestock Varieties to Meet the Evolving Needs of the NYS Potato Industry. 308. Faster Breeding of Vegetable Crops through Doubled-Haploid Techniques. 309. Corn Breeding and Sustainability. 310. Development of a Breeding Program to Improve the Non-Transgenic Resistance of Maize Against the Western Corn Rootworm. 311. Pigeonpea Breeding, Improvement, and Production. 312. Plant Genetic Resource Cultivation and Utilization. 313. Host Resistance as the Cornerstone for Managing Plant-Parasitic Nematodes in Sustainable Agroecosystems. 314. Evaluation and Improvement of Rice Germplasm for Texas. 315. Development of Epichloe Festucae as a Model System for Analysis of Fitness Enhancing Components of; Grass-Endophyte Mutualisms. 316. Breeding and Plant Development of Unique Geophytes. 317. Hard Red Spring Wheat Improvement. 318. Soybean Improvement via Classical and Molecular Breeding. 319. Breeding and Genetic Studies of Sweet Potato. 320. Development of Potato Cultivars for North Dakota Utilizing Germplasm Enhancement and Selection. 321. Development of New Potato Clones for Improved Pest Resistance, Marketability, and Sustainability in the East. 322. Environmental and Genetic Determinants of Seed Quality and Performance. 323. Potato Variety Selection, Evaluation, and Development. 324. Evaluation of Maize Germplasm, Hybrids, and Inbreds for Resistance to Gray Leaf Spot Disease under No-Tillage Production. 325. Pecan Breeding and Cultivar Evaluation. 326. Soybean Crop Improvement: Enhancing Nutrient Utilization. 327. Breeding Landscape Plants for Adaptation to Urban Environments. 328. Georgia Peanut Breeding and Genetics. 329. Genetic Improvement of Sour Cherry and Sweet Cherry Rootstocks. 330. Developing New Crops, Nutraceuticals, and other Value-Added Products. 331. Novel Breeding Methods, Genetic Enhancement, and Evaluation of Agronomic Crop Plants Focusing on Alfalfa (Medicago sp). 332. Floricultural Crop Breeding and Genetics for Plant Performance, Disease and Pest Resistance, and Stress Tolerance. 333. Wheat Genetic Improvement. 334. Breeding and Genetics of Spring Six-Rowed Barley for North Dakota. 335. Characterization and Commercialization of Wyoming-Bred Brown- Root- Rot-Resistant Alfalfa. 336. Breeding and Genetics of Hop. 337. Managing Karnal Bunt of Wheat. 338. Rice Breeding and Genetics. 339. Integration of Molecular and Classical Breeding for Turfgrass Improvement. 340. Sunflower Breeding and Testing Alternative Oilseed Crops for South Dakota. 341. Soybean Breeding, Genetics, and Production. 342. Developing Taro as an Alternative Food and Ornamental Crop. 343. Improvement of Edible Dry Bean. 344. Introduction and Evaluation of Ornamental Plants. 345. Introduction and Evaluation of Ornamental Plants. 346. Genetic Improvement of Native Plant Species for Coastal Restoration in Lousiana. 347. Developing Corn Silage Varieties with Improved Starch Utilization. 348. Development of Large-Fruited Early-Bearing Papaya in the Virgin Islands. 349. Identification of the Colonial Bentgrass Contribution to Dollar Spot Resistance in Colonial X Creeping Interspecific Hybrids. 350. Broadening the Genetic Base and Introgression of Resistance to Multiple Diseases in Pinto Bean. 351. Multistate Evaluation of Winegrape Cultivars and Clones. 352. Genetic Improvement of Cotton (Gossypium Hirsutum L.) Germplasm for New Mexico. 353. Genetic and Molecular Marker Strategies to Enhance Breeding for Multiple Disease Resistance in Maize. 354. Durum Wheat Improvement. 355. Development of Genetic Resources for Cotton. 356. Cotton Management Practices, Variety Choices for Quality, and Production Efficiency Improvements. 357. Genetic Improvement of Woody Plants (Trees and Shrubs) for Ornamental Uses. 358. Developing Black Raspberry for Diversified and Sustainable Agriculture Systems in the Northeast. 359. Small Grains Breeding and Genetics. 360. Breeding and Genetics of Spring Barley. 361. Developing Superior Oilseed and Mustard Cultivars for Brassicaceae. 362. Multistate Evaluation of Winegrape Cultivars and Clones. 363. Breeding Sorghum for Improved Yield Potential and Stress Tolerance. 364. Soybean Breeding. 365. Enhancement of Small Grains Productivity and Value by Breeding and Genetics. 366. Hard Winter Wheat Improvement. 367. Germplasm Enhancement, Breeding, and Genetics of Turf and Native Grasses. 368. Molecular Mapping and Marker-Assisted Selection and Breeding for Disease Resistance and Improved Fruit Quality in Tomato. 369. Improving Efficiency of Corn Breeding and Developing Alternative Breeding Methods. 370. DNA Marker-Assisted Small Grains Breeding. 371. Managing the Genetic Diversity of Michigan Pines. 372. Accelerating Domestication of Forest Trees for Intensive Plantation Forestry. 373. Genetics and Regeneration of Pennsylvania Hardwood Forests. 374. Evaluating Tree Varieties. 375. Development, Wilt Evaluation, and Marketing of Improved Seeds of Acacia Koa. 376. Genetic Structure within and among Four Grasses Native to Ponderosa Pine Ecosystems. 377. Genetic Improvement, Seed Orchard Construction, and Restoration of Tennessee Forest Species. 378. Biological Improvement, Habitat Restoration, and Horticultural Development of Chestnut by Management of Populations,; Pathogens, and Pests. 379. Forest Genetics Research for Sustainable Forest Improvement in Mississippi. 380. Quantitative Genetics and Tree Improvement of Southern Pines. 381. Genetics of Wheat Grain Hardness Genes. 382. QTL Mapping and Population Structure of Insecticide Resistance in Corn Rootworm. 383. Genetic Dissection of Quantitative Resistance Using the Barley: Barley Stripe Rust Model. 384. Genetic Control of High Oleic Acid Seed Content in Soybean. 385. QTL Dissection of Variance Sources for Long-Term Selection. 386. Simulation Modeling of Heading Time in Rice: A Genetic Control Network Approach. 387. Raspberry as a Model System for Studying Phytophthora Root Rot Resistance and for Testing Marker-Assisted; Selection in Cultivar Development. 388. Genetic Correlates of Weediness in Cereal Rye (Secale Cereale). 389. Development of Corn Germplasm to Reduce Aflatoxin Contamination and Genetic Characterization of; Aflatoxin Resistance. 390. New Genetic Approach to Wide-Species Hybridization, Detection of Alien Chromatin, and Transfer of; Agronomically Important Genes into Sorghum. 391. Comparative Analysis of Phenotypic and Marker-Assisted Selection in Cucumber for Multiple Traits. 392. Conference Planning Proposal for a 2005 Coordinated Agricultural Project (CAP) in Wheat Translational Genomics. 393. A Coordinated Research, Education, and Extension Project for the Application of Genomic Discoveries to Improve; Rice in the United States. 394. CAP Conference Proposal: Translational Genomics for Cotton. 395. Coordinated Agricultural Project Conference on Barley Translational Genomics. 396. APGI-CAP Conference: Soybean Translational Genomics. 397. Genecology, Genetic Diversity, and Adaptive Trait Variation in Bitterbrush (Purshia Tridentata) from the Pacific Northwest. 398. Feed and Forage Analyzer 6500 Equipment Grant. 399. Genetic Diversity of Wild Apple Accessions in the National Plant Germplasm System. 400. Gene Pair Haplotypes and Sequence Samples from Strawberry (Rosaceae): Multipurpose, Transferable Resources; for Genomics and Variety Improvement. 401. Development of Segregating Populations for Molecular and Genetic Analyses of X-Disease in Chokecherry; (Prunus Virginiana L.) 402. Application of Genetic Approaches to Enhance Cold-Hardiness of Guava. 403. Characterizing Cowpea Genotypes for Drought Tolerance in the Delmarva Ecosystem. 404. Bringing Genomics to the Wheat Fields. 405. Assessment of Weediness and Fertility of Hybrids between Creeping Bentgrass and Related Species. 406. Interdisciplinary Training Program in Agricultural Biology: Linking Emerging and Existing Technologies. 407. Educating Young Researchers for Sustainable Agriculturally-Based Bio-Industries. 408. Harnessing Investments in Genomics of Model Species for Vegetable Improvement. 409. Establishment of a Grape (Vitis L.) Germplasm Center with Emphasis on Evaluation and Genetic Analysis of Pierce's Disease. 410. Identification and Characterization of Potato Clones for Organic Production Systems. 411. Southern Regional Canola Research Program. 412. Development of Seedless Pawpaw Fruit by Germplasm Enhancement. 413. Development and Management of Canola in the Great Plains Region. 414. Utilization of Genomics for Molecular Breeding of High-quality and Disease-Resistant Peppers. 415. Biological Nitrogen-Fixation and Seed-Composition Traits of White Lupin. 416. Southern Regional Canola Research Program. 417. Stakeholder Workshop Implementation of Molecular Marker Technologies in Public Wheat Breeding Programs. 418. Natural Systems Agriculture. 419. Gene Flow in Transgenic Tall Fescue and Ryegrasses: Pollen Dispersal and Hybridization Potential with Related Grass Species. 420. Use of Resident Biological Resources for the Management of Replant Disease in Organic Tree Fruit Production Systems. 421. The Organic Seed Partnership. 422. International Cotton Research Center. 423. North Central Region Canola Research Program. 424. Southern Regional Canola Research Program. 425. International Cotton Research Center--Part 3 (Breeding and Genetics Projects). 426. Developing Medicinally Used Echinacea Cultivars by Intra-and Inter- Species Hybridization. 427. Improved Agricultural Sustainability through Microbial Enhanced Disease Resistance and Yield in Corn. 428. Developing Medicinally Used Echinacea Cultivars by Intra-and Inter- Specific Hybridization--Phase II. 429. Biology, Epidemiology, and Development of Methods for Detection and Suppression of Citrus Canker. 430. Using A Patho-System Approach to Develop Disease-Resistant Ornamental Foliage Plants. 431. Squash Breeding for Disease Resistance to Phytophthora Blight, Caused by P. Capsici. 432. Environmental Constraints and Genetic Improvement of Tropical Forage Production. 433. Improving the Sustainable Production of Specialty Crops. 434. Alternative Crops for Arid Lands. 435. Technology and Market Development for the Gulf Coast Satsuma Mandarin Industry. 436. Crop Diversification, North Dakota and Missouri. 437. Hawaii Floriculture Research Grant--2002. 438. Tropical and Subtropical Agricultural Research (T-STAR) for Hawaii 2002: Umbrella A. 439. Hawaii Agricultural Diversification 2002--New Crop/Product Development for Market Niches. 440. Caribbean Basin Tropical and Subtropical Agriculture Research-- Virgin Islands. 441. T-STAR Agricultural Research at the University of Guam for FY2002. 442. Identification and Introgression of Silverleaf Whitefly (Bemissa Argentifolii) Resistance Genes from; Lycopersicon Hirsutum to Tomato. 443. Integrated Biotechnological and Genetic Systems for Enhanced Forest Productivity and Health. 444. Improving the Sustainable Production of Specialty Crops. 445. Northwest Center for Small Fruits Research Program. 446. Peach Tree Short Life in South Carolina. 447. Genetically Enhancing the Industrial Oilseed Crop Meadowfoam. 448. Blueberry and Cranberry Breeding, Disease, and Insect Management. 449. Management of Russian Wheat Aphids in Dryland Cropping Systems of the Great Plains. 450. International Cooperation for Agricultural Research in Central Asia and the Caucasus. 451. Technology and Market Development for the Gulf Coast Satsuma Mandarin Industry. 452. Tropical and Subtropical Agricultural Research (T-STAR) Umbrella B: Tropical Agriculture. 453. Tropical and Subtropical Agricultural Research (T-STAR) Umbrella D: Agriculture Research in the Tropics. 454. Hawaii Floriculture Research Grant--2003. 455. Caribbean Basin Tropical and Subtropical Agriculture Research-- Virgin Islands. 456. Life and Death in Plants: Studies on Perennial Wheat as a Sustainable Alternative Cropping System. 457. Developing Multi-Species Insect Resistance in Romaine Lettuce. 458. Galia Melon: A New High-quality Shipping Melon for Florida Producers. 459. Genetic Diversity and Domestication of Forage Legumes for the Subtropics and Tropics. 460. Development of Phytophthora Root Rot-Resistant Avocado Rootstocks for the Caribbean. 461. Peach Tree Short Life in South Carolina. 462. Controlling Fire Blight of Apple Trees. 463. Potato Breeding and Variety Development to Enhance Pest Resistance and Marketing Opportunities in the; Eastern United States. 464. Potato Variety Development and Improvement in the Northwest. 465. Development of Multipurpose Potato Cultivars with Enhanced Quality, Disease, and Pest Resistance--North; Central Program. 466. Northwest Center for Small Fruits Research Program. 467. International Cooperation for Agricultural Research in Central Asia and the Caucasus. 468. Improving the Sustainable Production of Specialty Crops. 469. Potato Breeding and Cultivar Development in the Southwest. 470. Identification, Inheritance, and Utilization of Host Plant Resistance in Caladiums to Fusarium and Pythium. 471. Genetic Control of Ripening of West Indian and West Indian- Guatemalan Avocado Fruit. 472. Squash Breeding for Disease Resistance to Phytohthora Blight Caused by P. Capsici Part II. 473. Identification and Introgression of Silverleaf Whitefly (Bemisia Argentifolii) Resistance Genes from; Lycopersicon Hirsutum to Tomato. 474. Grass Seed Cropping Systems for a Sustainable Agriculture: ID, OR, and WA. 475. Development of Citrus Germplasm that Will Eliminate Loss of Trees and Production Due to CTV. 476. Increasing Sustainability of Tropical Pastures through Selection of Legumes Tolerant to Drought and Aluminum. 477. Hawaii Floriculture Research Grant--2004. 478. Feed Barley for Rangeland Cattle. 479. Varietal Variation in Papaya Fruit Softening and Its Inheritance. 480. Tropical and Subtropical Agriculture Reseach (T-STAR) Umbrella B- -2004. 481. Improving the Sustainable Production of Specialty Crops. 482. Center for Sorghum Improvement. 483. Designing Foods for Health. 484. Potato Breeding and Variety Development to Enhance Pest Resistance and Marketing Opportunities in the; Eastern United States. 485. Novel Approaches to Integrated Management of Armillaria Root Rot of Peach. 486. Blueberry and Cranberry Breeding, Disease, and Insect Management. 487. International Cooperation for Agricultural Research in Central Asia and the Caucasus. 488. Field Testing of Resistant Tomato Lines to Control Late Blight and Early Blight in Conventional and Organic Growing Systems. 489. Northwest Center for Small Fruits Research Program. 490. Peach Tree Short Life in South Carolina. 491. Organic Cropping Research for the Northwest. 492. Developing Multi-Species Insect Resistance in Romaine Lettuce. 493. Screening Tropical Pumpkin and Related Species for Melonworm Resistance. 494. Enhancing the Genetics and Productivity of the Oilseed Crop Meadowfoam. 495. Genetic Diversity and Domestication of Forage Legumes for the Subtropics and Tropics. 496. Molecular Improvement of Physiological Traits Defining the Environmental Adaptation of Tropical Forage; Grass Production. 497. Improving the Quality of Kava Beverage. 498. Integrated Management of Phytophthora Root Rot of Avocado in Puerto Rico. CSREES provided a list of current extramural classical animal breeding projects. Projects with funding to multiple states may be repeated on this list. 1. Advanced Technologies For the Genetic Improvement of Poultry. 2. Alabama Beef Connection. 3. Analysis of the Hairless-Wrinkled Mouse: A Spontaneous Mutant with Severe Skin Abnormalities. 4. Application of Genomic and Proteomic Approaches to the Improvement of Disease Resistance and Performance in; Farm Animals. 5. Assessment of Live Animal, Quantitative Genetic, and Molecular Biological Approaches to Enhance Genetic; Improvement in Pork Quality. 6. Association of Imprinted Genes with Reproductive Efficiency in Swine. 7. Beef Cattle Breeding and Management. 8. Beef Cattle Breeding at the V Bar V Ranch. 9. Beef Cattle Production Systems in the Southern Great Plains. 10. Biological Basis for Variation in Net Feed Efficiency in Beef Cattle. 11. Cell Cycle Control of Mouse Embryonic Stem Cells. 12. Cell-Mediated Gene Transfer in Fish. 13. Cellular and Molecular Characterization of the Spider Lamb Syndrome, a Heritable Chondrodysplasia. 14. Characterization and Genetic Evaluation of Conformation and Gait in the American Warmblood and Tennessee; Walking Horse. 15. Characterization of a Unique Ap4A Receptor. 16. Comparative Mapping of the Bovine Genome. 17. Controlled Breeding, Larviculture, and Intensive Growout of High- value Marine Fish Species for U.S. Agriculture. 18. Delineation of Interactive Molecular and Genetic Mechanisms Involved in Pathogenesis Disease Using Unique Modalities as; Investigative Probes. 19. Developing New Technology for Aquaculture in Louisiana. 20. Development of Selection and Mating Strategies to Improve Dairy Cattle Health and Performance Using Field Data. 21. Discovery and Evaluation of Genetic Factors that Influence Growth, Carcass Merit, and Meat Quality of the Pig. 22. Discovery and Use of Quantitative Trait Loci Associated With Growth, Carcass Traits, and Feed Efficiency in Beef Cattle. 23. Effect of Culture Conditions on the Protein Expression Patterns and Viability of Bovine Embryos. 24. Effect on Carcass Traits Due to Sire Selection Based on EPD Predicted From Live Animal Carcass Measures; On Young Seedstock. 25. Effects of Genotype and Plane of Nutrition Performance, Carcass Composition, and Meat Quality Traits of; Guinea Fowl (Numida Meleagris). 26. Engineering Mammalian Glutamine Metabolism. 27. Enhancement of Dietary Energy Use For Maintenance, Growth, and Lactation by Beef Cattle. 28. Evaluating and Modeling Extended Lactations in Dairy Goats. 29. Evaluation and Further Development of Sheep Genetic Resources. 30. Evaluation of Crossbred Calf and Cow Types for the Coastal Plan of North Carolina. 31. Evaluation of Market Potential and Production Characters Related to Two Specialty Markets for Maine Livestock Producers. 32. Expression and Function of Chicken MHC Class I Molecules. 33. Factors Mediating Nuclear Reprogramming in Porcine Embryos Produced by Using Nuclear Transfer. 34. Feed Efficiency in Cattle. 35. Finfish Aquaculture: Improved Production Technologies, Cultivars, and Farming Practices. 36. Genetic (Co) Variance of Parasite Resistance, Temperament, and Production Traits of Traditional and Non-Bos Indicus Tropically Adapted Breed. 37. Genetic (Co) Variance of Parasite Resistance, Temperament, and Production Traits of Traditional and Non-Bos Indicus Tropically Adapted Breeds. 38. Genetic (Co)Variance of Parasite Resistance, Temperament, and Production Traits of Traditional and Non-Bos Indicus Tropically Adapted Breed. 39. Genetic Analysis of Fatty Acid Composition of Beef and Milk- Developing Tools for Use in Selection. 40. Genetic Analysis of Production Traits in Beef Cattle and Sheep. 41. Genetic Analysis of Selected Traits in Swine. 42. Genetic and Environmental Aspects of Dairy Cattle Health and Milk Quality. 43. Genetic and Functional Genomic Approaches to Improve Production and Quality of Pork. 44. Genetic and Functional Genomic Approaches to Improve Production and Quality of Pork. 45. Genetic Approaches to Enhance Efficiency and Profitability of Pork Production. 46. Genetic Bases for Resistance and Immunity to Avian Diseases. 47. Genetic Effects on Reproduction in Beef Cattle. 48. Genetic Engineering of Dairy Animals to Improve Milk Composition. 49. Genetic Enhancement of Agriculturally Important Animals. 50. Genetic Improvement of Aquaculture Stocks. 51. Genetic Selection and Crossbreeding to Enhance Reproduction and Survival of Dairy Cattle. 52. Genetic Selection and Crossbreeding to Enhance Reproduction and Survival of Dairy Cattle. 53. Genetic Selection and Crossbreeding to Enhance Reproduction and Survival of Dairy Cattle. 54. Genetic Variation in Feed Energy Utilization. 55. Genetic, Nutritional, and Environmental Methods to Improve Hatchability in Long-Stored Avian Eggs. 56. Genetics and Functional Genomic Approaches to Improve Production and Quality of Pork. 57. Genetics of Growth and Reproduction in Rainbow Trout (Oncorhynchus Mykiss) Fed a Plant-Based Diet. 58. Genetics of Growth and Reproduction in the Turkey. 59. Genetics of Phytate Phosphorus Utilization in Chickens. 60. Genetics Selection for Increased Hatchability of Japanese Quail Embryos when Incubated at 102°F. 61. Germ Cell and Embryo Development and Manipulation for the Improvement of Livestock. 62. Growth-Hormone Receptor DNA Polymorphisms and Their Associations with Growth Traits in Grass-Fed Cattle Populations. 63. Haplotype Structure of the Bovine Prion Gene Region and Association with Bovine Spongiform Encephalopathy. 64. Hawaii Agricultural Diversification 2005--New Crop/Product Development and Marketing. 65. Homologous Gene Targeting of Primary Embryonic Bovine Fibroblast Cells. 66. Identification of Genes Controlling Animal Growth and Development. 67. Identification of Genes Underlying Production Traits in Poultry. 68. Identification of the Cellular Components Involved in the Recognition and Pairing of Homologous Chromosomes during Meiosis. 69. Improved Ewe Productivity. 70. Improving Nuclear Transfer Efficiency Using Donor Cells of Known XCI Patterns. 71. Improving Responses of Range Beef Cattle to Estrus Synchronization. 72. Improving the Design Of Breeding Schemes in Ruminant Livestock Using a Sheep Paradigm. 73. Improving Yields of Pacific Oysters through Selection. 74. Increasing Sheep Productive Efficiency. 75. Inflammatory Responses to Diseases. 76. Integrated Resource Management Beef Production Systems for the South Carolina Coastal Plains. 77. Interpreting Cattle Genomic Data: Biology, Application, and Outreach. 78. Interpreting Cattle Genomic Data: Biology, Applications, and Outreach. 79. Interpreting Cattle Genomic Data: Biology, Applications, and Outreach. 80. Livestock Management Systems. 81. Maintenance of Immune Gene Variation. 82. Material Genotype and Fescue Endophyte Effects on Meat Goats' Performance Traits. 83. Metabolic Profiling of Butterball, a Morbidly Obese Mouse Mode. 84. Minimizing Neonatal Lamb Losses. 85. Molecular and Cellular Mechanisms in Agriculture. 86. Molecular and Cellular Mechanisms in Agriculture: Gene Expression Mechanisms. 87. Molecular Cloning and Characterization of the Androgenic Hormone(s) in Aquacultured Prawns and Shrimp. 88. Molecular Mechanisms Regulating Skeletal Muscle Growth and Differentiation. 89. Multi-Cropping Strategies for Aquaculture: A Collaborative Approach to Aquaculture Research and Extension--2004. 90. Multi-Cropping Strategies for Aquaculture: A Collaborative Approach to Aquaculture Research and Extension--2005. 91. Muscularity Genes and Their Functional Regulations for Efficient Animal Production. 92. National Animal Genome Research Program. 93. National Animal Genome Research Program (From NSRP-8). 94. National Animal Genome Research Program Species Coordinator for the Horse. 95. National Animal Genome Research Project (NRSP-8): Aquaculture Genomics (Oysters). 96. National Beef Cattle Genetic Evaluation. 97. NC1010: Interpreting Cattle Genomic Data: Biology, Applications, and Outreach (NC-209). 98. Nutritional and Genetic Factors Affecting Growth, Adipocyte Development, and Muscle Characteristics in Cattle. 99. Ohio Aquaculture Research and Development Initiatives. 100. Paternal, Maternal, and Environmental Influence on Hatchability and Post-Hatching Survival of Turkeys. 101. Performance of Crossbred Hair Sheep Ewes under Extensive Management in the Tropics. 102. Physiological Genomics of Growth and Reproduction in Chickens. 103. Poultry Production Systems: Optimization of Production and Welfare Using Physiological, Behavioral, and Physical Assessments. 104. Predicting Genetic Merit by Gene-Expression Profiling. 105. Regional Aquaculture Center. 106. Reproductive Physiology in Male Japanese Quail Selected for Divergent Adrenocortical Responsiveness to Restraint. 107. Risk Assessment of a-Lactalbumin Transgenic Pigs. 108. Role of Antioxidants in Health and Disease in Poultry. 109. Roles of a GTPase-Activating Protein, PRGAP, in Mediating Pitx2 Function. 110. Selection Strategies for Improving the Pulmonary Vascular Capacity in Broilers. 111. Selective Breeding Programs for Commercially Important Bivalves in Maine. 112. Shellfish Genetics and Breeding for Aquaculture. 113. Statistical Procedures for Genetic Evaluation of Susceptibility to Mastitis in Dairy Cattle. 114. Strategies to Enhance Meat Goat Production in North Carolina. 115. Systems Evaluation of Animal Production. 116. The Genomic and Proteomic Basis of Marek's Disease Virus-Induced Cellular Transformation. 117. The Interface of Molecular and Quantitative Genetics in Plant and Animal Breeding. 118. The Mechanism of PDHE1/Fdi Regulation in Response to Oxidative Stress in Azotobacter Vinelandii. 119. The Molluscan Broodstock Program. 120. The Relationship between Function and Mutations within the Mitochondrial NADH Dehydrogenase Complex 1 Gene. 121. Tn5 Transposase--Host-Protein Interaction. 122. Understanding Ecological Aspects of Shellfish Pathogens to Improve Management. 123. Uracil-DNA Repair in Vitro and in Vivo. 124. Use of Halothane Gas to Identify Novel SR Calcium Release Channel Protein Defects in Pigs. 125. Utilization, Characterization, and Preservation of Goat Genetic Resources II. 126. Validation and Characterization of a High-density Chicken SNP Map. 127. Western Regional Aquaculture Center--17th Annual Work Plan (FY03). 128. Western Regional Aquaculture Center--18th Annual Work Plan (FY04). 129. Western Regional Aquaculture Center--19th Annual Work Plan (FY05). [End of section] Footnotes: [1] For the purposes of this report, USDA defines a scientist-year as a full-time, permanent scientist assigned to the program. [2] Genomics refers to the study of genes and their function. Genetic engineering refers to methods by which biologists splice genes from one species into the DNA of another species in an attempt to transfer chosen genetic traits. [3] Extramural research refers to research that is funded by federal sources but conducted by nonfederal entities. [4] For the purposes of this report, USDA defines plant germplasm as the genetic variation of a species and animal germplasm as any unique breed, line, or strain of a species. [5] Dollar figures have been adjusted for inflation to 2007 dollars. [6] CSREES noted that it also contributes to the infrastructure for classical plant breeding in the United States through funding for plant breeding research at state agricultural experiment stations and land- grant universities, including funding for collaborative work with NPGS. [7] Pub. L. No. 96-517, § 6, 94 Stat. 3019 (1980). GAO's Mission: The Government Accountability Office, the audit, evaluation and investigative arm of Congress, exists to support Congress in meeting its constitutional responsibilities and to help improve the performance and accountability of the federal government for the American people. 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